Microsurgical Testis Sperm Extraction Testis Sperm Extraction (TESE) is carried out in those unfortunate men who have a problem with sperm production to such a degree that there are no sperm seen in the semen. By microsurgically searching through the testis, I look for areas that may have tiny amounts of sperm within them. Those areas are harvested and either used right away in an ICSI cycle or frozen for later use. As for MESA, we first described the intentional frozen-thawed approach, now used world-wide. A karyotype and Y chromosomal microdeletion assay almost always need to be obtained prior to TESE. Dr Mahtab Singh Best Sexologist In jaipur Urologist, Andrologist, Sexologist, Male Infertility. Experience: 15 Years. Education: MBBS, MS General Surgery, Mch Urology, DrNB Urology